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Tetracyline


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4 replies to this topic

#1 samita

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Posted 02 March 2011 - 10:43 AM

What is the stock concentration of tetracyline and what is recommended concentration for E. Coli. I made the stock of 15mg/ml is ethanol. It does not dissolve fully, even after vibrating it as well. How to dissolve it.
regards

#2 pDNA

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Posted 02 March 2011 - 11:19 AM

you have to be careful what you are using! ...if you use Tetracycline Hydrochloride this one is only slightly soluble in ethanol ...so be careful and check what powder you are using. If you use Tetracycline it should be soluble in ethanol ...i make the stocks in 70%EtOH at a concentration of 5 mg/mL ...working concentration in E. coli is about 15-20 g/mL.

Good luck!

Regards,
p

What is the stock concentration of tetracyline and what is recommended concentration for E. Coli. I made the stock of 15mg/ml is ethanol. It does not dissolve fully, even after vibrating it as well. How to dissolve it.
regards



#3 pito

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Posted 02 March 2011 - 11:27 AM

15mg/ml is ok as a stock concentration.
In the end you should have a working concentration of about 10-20µg/ml

you should try 50% ethanol.

(are you sure its tetracycline? and not tetracycling-HCl?)

But I see pDNA has allready told you that, I was too slow. :rolleyes:

Edited by pito, 02 March 2011 - 11:36 AM.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#4 samita

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Posted 02 March 2011 - 12:21 PM

thanks for your kind reply. I will try now 70 percent ethanol. I think Tet-Hcl powder....

#5 pito

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Posted 02 March 2011 - 12:41 PM

thanks for your kind reply. I will try now 70 percent ethanol. I think Tet-Hcl powder....

That explains it. Tet-HCl doesnt dissolve very well in ethanol.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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