Posted 02 March 2011 - 10:43 AM
Posted 02 March 2011 - 11:19 AM
What is the stock concentration of tetracyline and what is recommended concentration for E. Coli. I made the stock of 15mg/ml is ethanol. It does not dissolve fully, even after vibrating it as well. How to dissolve it.
Posted 02 March 2011 - 11:27 AM
In the end you should have a working concentration of about 10-20µg/ml
you should try 50% ethanol.
(are you sure its tetracycline? and not tetracycling-HCl?)
But I see pDNA has allready told you that, I was too slow.
Edited by pito, 02 March 2011 - 11:36 AM.
Posted 02 March 2011 - 12:21 PM
Posted 02 March 2011 - 12:41 PM
That explains it. Tet-HCl doesnt dissolve very well in ethanol.
thanks for your kind reply. I will try now 70 percent ethanol. I think Tet-Hcl powder....