Amino acid starvation help
Posted 02 March 2011 - 06:10 AM
Posted 02 March 2011 - 02:53 PM
Posted 06 March 2011 - 12:05 PM
FBS contains all sorts of stuff, including proteins which the cells can break down into component AAs...
If you think you really need to use FBS, consider dialyzing the serum against PBS. We do that in cells that we label for pulsed SILAC