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superoxide measurement


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#1 Cindy Pidgeon

Cindy Pidgeon

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Posted 25 May 2002 - 05:36 AM

Hi all!  I was wondering if anyone has had problems using diethyldithiocarbamate as a SOD inhibitor in the lucigenin assay for superoxide?  DDC seems to only be effective in my system for about 2 hours then precipitates.  Does anyone know of an alternative SOD inhibitor and/or superoxide detection method other than ESR?

#2 kishorebio

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Posted 02 September 2003 - 06:42 AM

Hi
U can go for Cytochrome C reduction method to measure Superoxide oins.
Want more i can provide u later




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