Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

IHC - really tough wax!!


  • Please log in to reply
2 replies to this topic

#1 steffi333

steffi333

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 01 March 2011 - 03:43 AM

Hello,

I recently got some slides from another lab, and I just cannot get the wax off of them! :blink: I've emailed them asking for help but have yet to hear back from them, so thought somebody on here might have some answers.

Normally we dewax with a series of three histoclear washes followed by 2 abs EtOH washes and one 70% EtOH wash. This didn't work... so we put the slide in a 60 degree oven for about an hour.... wax still didn't budge... didn't even melt in the slightest! And now the tissue isn't looking very happy either. :(

Does anybody have any experience with hardcore wax that won't budge?

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,758 posts
130
Excellent

Posted 01 March 2011 - 08:50 AM

you may have to bite the bullet and use xylenes or you could try histosol instead of histoclear.
talent does what it can
genius does what it must
i do what i get paid to do

#3 steffi333

steffi333

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 07 March 2011 - 04:21 AM

Fixed the problem. Trick was to heat slides in a 60 degC oven for circa 1 hr, then place in histoclear IMMEDIATELY and shake for about 5 five mins. Then proceed with the remaining histoclear washes and ethanol etc.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.