IHC - really tough wax!!
Posted 01 March 2011 - 03:43 AM
I recently got some slides from another lab, and I just cannot get the wax off of them! I've emailed them asking for help but have yet to hear back from them, so thought somebody on here might have some answers.
Normally we dewax with a series of three histoclear washes followed by 2 abs EtOH washes and one 70% EtOH wash. This didn't work... so we put the slide in a 60 degree oven for about an hour.... wax still didn't budge... didn't even melt in the slightest! And now the tissue isn't looking very happy either.
Does anybody have any experience with hardcore wax that won't budge?
Posted 01 March 2011 - 08:50 AM
genius does what it must
i do what i get paid to do
Posted 07 March 2011 - 04:21 AM