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IHC - really tough wax!!

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#1 steffi333



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Posted 01 March 2011 - 03:43 AM


I recently got some slides from another lab, and I just cannot get the wax off of them! :blink: I've emailed them asking for help but have yet to hear back from them, so thought somebody on here might have some answers.

Normally we dewax with a series of three histoclear washes followed by 2 abs EtOH washes and one 70% EtOH wash. This didn't work... so we put the slide in a 60 degree oven for about an hour.... wax still didn't budge... didn't even melt in the slightest! And now the tissue isn't looking very happy either. :(

Does anybody have any experience with hardcore wax that won't budge?

#2 mdfenko


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Posted 01 March 2011 - 08:50 AM

you may have to bite the bullet and use xylenes or you could try [url="[url]http://www.nationaldiagnostics.com/product_info.php/cPath/24_41/products_id/179"]histosol[/url][/url] instead of histoclear.
talent does what it can
genius does what it must
i do what i get paid to do

#3 steffi333



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Posted 07 March 2011 - 04:21 AM

Fixed the problem. Trick was to heat slides in a 60 degC oven for circa 1 hr, then place in histoclear IMMEDIATELY and shake for about 5 five mins. Then proceed with the remaining histoclear washes and ethanol etc.

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