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[olga_m]

Hello everyone!

I am trying to establish a co-culture system of lung epithelial cells and fibroblasts using transwells with pore diameter 1μΜ and 0.4μΜ. I wonder if there is a different protocol for lysis of the cells grown on inserts.
In the case of plain cell culture dishes i used to wash the cells twice with ice cold PBS and then extract the total proteins by scrapping using the appropriate lysis buffer. Can I use the scrapper on the membrane of the insert or there is a danger to damage it? Is it possible to loose any buffer or protein extract through the transwell pores?
I would appreciate any advice very much!

Thank u!!!