Are the last 2 lanes degraded RNA? What bout the 1st four lane? Is my separation time too short? Samples are incubated with formamide+formaldehyde at 65C for 15 minutes before loading.
Uploaded with ImageShack.us
This gel is a bit weird because the bands are of length 2000bp and 1000bp... Is it because i didn't add and incubate the RNA with formamide+formaldehyde?
Edited by hianghao, 01 March 2011 - 03:25 AM.