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Phages concentration and purirication


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#1 BHY

BHY

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Posted 28 February 2011 - 08:31 PM

Hi, now I work on isolating phages from water. I already success to get a strain of phages but I would like to concentrate and purified it before I do TEM. Is it good enough to use Millipore Amicon 30kDa to concentrate and purified the lysate (after filter 0.2 um)? Or better use PEG/NaCl method? If I just concentrate the phages without vertrelor/ chloroform treatment (purification)is it okay for phages morphology study? Thanks

Edited by BHY, 28 February 2011 - 08:32 PM.





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