cells dont attach hard enough to 96 well
Posted 28 February 2011 - 01:24 PM
I have immortalized cells that grow rapidly in a flask and are attached strongly. For an XTT assay I grow them in 96 well. I seed 3000 cells per well. After 24 hr I wash them with PBS and change medium. I notice a lot of the cells (majority)are washed away during this process. Cells remain in the center and near the walls.. In the beginning they spred evenly. Could you advice me what can I try to attach them better?
Thank you so much
Posted 01 March 2011 - 02:42 PM
You could try coating the plate with gelatine (collagen), poly-L-lysine, fibronectin...
Posted 20 December 2011 - 10:34 AM
Also, I heard people use PBS with Ca++ Mg++ to wash gentle cells, you can read the relevant discussion here http://www.protocol-...-or-wout-ca-mg/
Posted 09 January 2012 - 07:01 AM