Hello,
I found this protocol on google. It basically skip the gel extraction step, and directly use the DNA that was still in agarose.
http://neuroscience....cesF/clone.html
Anyone has tried this please let me know. I am in the process of finding a good strategy for my cloning, I havent started anything yet. Is this shortcut work ?
Thank you,
0
3 replies to this topic
#2
-
- Active Members
-
- 3,047 posts
Hmmm, I think it's working
Posted 27 February 2011 - 03:30 PM
I know people who do it, and it seems to work OK.
#3
-
- Active Members
-
- 13 posts
member
Posted 28 February 2011 - 04:17 PM
so using this shorcut, you would ignore the calculation part ... no more molar ratio btw plasmid and insert? I d like it if it does work
#4
-
- Active Members
-
- 3,047 posts
Hmmm, I think it's working
Posted 28 February 2011 - 04:25 PM
I guess, though you could calculate the molar ratio given the amount of input...
