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Comment of this cloning strategy


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3 replies to this topic

#1 stagius24

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Posted 26 February 2011 - 11:22 PM

Hello,

I found this protocol on google. It basically skip the gel extraction step, and directly use the DNA that was still in agarose.
http://neuroscience....cesF/clone.html

Anyone has tried this please let me know. I am in the process of finding a good strategy for my cloning, I havent started anything yet. Is this shortcut work ?

Thank you,

#2 bob1

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Posted 27 February 2011 - 03:30 PM

I know people who do it, and it seems to work OK.

#3 stagius24

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Posted 28 February 2011 - 04:17 PM

so using this shorcut, you would ignore the calculation part ... no more molar ratio btw plasmid and insert? I d like it if it does work

#4 bob1

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Posted 28 February 2011 - 04:25 PM

I guess, though you could calculate the molar ratio given the amount of input...




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