I'm trying to find a protocol for preparation of mRNA for transfection into cells but all I can find is stuff about preparing probes. These mRNAs need to be polyadenylated and capped so they don't get degraded. Do I just follow the same protocol as would make a probe and then add on to that a polyadenylation/capping protocol?
How to make mRNA in vitro for subsequent transfection?
Started by seanspotatobusiness, Feb 26 2011 03:56 PM
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