HepG2 cells culture conditions
Posted 26 February 2011 - 11:14 AM
I'm gonna start to culture HepG2 cells, but I'm a little confuse because in the literature that I have reviwed people used many types of medium (DMEM, RPMI and MEM). What is the best medium for this type of cells?? And, it is necessary use Glutamine??
Posted 27 February 2011 - 03:46 PM
Glutamine is necessary most of the time for healthy growth. You should add this to your medium fresh each time you open a bottle.
Posted 04 March 2011 - 07:08 PM
I culture them in DMEM with glutamine, pyruvate and 10% FBS....
But yeah, you must check in which medium they have been growing...