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Chloroplast DNA isolation


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#1 Tucker93

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Posted 22 March 2002 - 06:33 AM

I am interested if anyone has a method to isolate cpDNA that they know works. Currently I am using an isopycnic/CsCL high speed centrifugation method that does not yield much DNA? I am trying to extract samples enriched for cpDNA from Chlamydomonas (green algae).

#2 Sciad

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Posted 10 July 2002 - 08:29 AM

Hi,
though I don't have personal experience with your organism, just a thought:
Can you disrupt the algae in such a way that the chloroplast remains mostly intact? If so, you could try separating them by centrifugation from the cellular material.




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