4 replies to this topic

[tonghuitong]

I have one question confused me. I culture insulinoma cell line INS1 cell, When I treated the cells with low or high concentration of glucose, I use 1640 medium contain 1% FBS, and the cells grow well, and the result is nice. (also I set control, cultured with 1%FBS medium too).  I am not sure whether the data are persuasive?      
      Because the cells grow very fast, and I usually need treat cells for 2 days, So I use this medium to prevent their fast growing and keep the concentration of glucose in medium constant.  
      When the cells were treated by one factor, Should we use normal(10%FBS) medium or low serum medium(1%FBS  )?

[bob1]

If this is used for comparison to your high/low glucose experiments, you should keep the FCS the same as in those experiments i.e. 1%.

[tonghuitong]

Thank you! If my result is nice, whether that suggest it is also the case in vivo?  

And Whether you means that if I use 10%FBS, I can still get similar results, but may be not better than this one?

[bob1]

No, in-vitro work suggests very little about in-vivo situations as there are all sorts of things going on in the body that will not be present in cell culture.

You may be able to get similar results with 10%.  However, if you are comparing the two results, according to the scientific method, you should only change one variable at a time so as to remove the possibility that the second variable is not causing the effect.  Also, you can't compare systems with hugely different components, as things will behave differently in each one.

[tonghuitong]

thank you very much, Best wishes to you and your research...