Should I use fully supplymented medium or low serum medium when treat INS1 cells
Posted 25 February 2011 - 10:18 PM
Because the cells grow very fast, and I usually need treat cells for 2 days, So I use this medium to prevent their fast growing and keep the concentration of glucose in medium constant.
When the cells were treated by one factor, Should we use normal(10%FBS) medium or low serum medium(1%FBS )?
Posted 27 February 2011 - 03:48 PM
Posted 27 February 2011 - 07:00 PM
And Whether you means that if I use 10%FBS, I can still get similar results, but may be not better than this one?
Posted 28 February 2011 - 04:11 PM
You may be able to get similar results with 10%. However, if you are comparing the two results, according to the scientific method, you should only change one variable at a time so as to remove the possibility that the second variable is not causing the effect. Also, you can't compare systems with hugely different components, as things will behave differently in each one.
Posted 28 February 2011 - 07:11 PM