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Bleeding during i.p. injection


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5 replies to this topic

#1 Limpa

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Posted 25 February 2011 - 08:24 AM

During IP injection, my mouse had a bit of bleeding. What does that mean? Will it die or is it going to mess up my experiment?

#2 bob1

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Posted 25 February 2011 - 03:58 PM

You hit a vein or artery... it was not correctly injected and it may affect the experiment. As to whether the animal will survive, it depends on how big an injury you did (e.g. did you hit the renal artery or a minor vein?) and what was being injected.

#3 Limpa

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Posted 25 February 2011 - 04:17 PM

Rapamycin was injected. There was one small drop of blood, so may not be huge injury?

#4 pito

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Posted 26 February 2011 - 03:18 AM

Rapamycin was injected. There was one small drop of blood, so may not be huge injury?


In small animals even the loss of 2ml (or less) can be bad.
Another thing is that you cant see what happened internally: bleeding internally, wounding, etc..

Now changes are big that its not a big problem.
However as bob said: the changes are that the experiment failed. Its possible you for example lost the antibioticum with the blood that got lost.

Anyway: normally there should be no blood when injecting the mouse.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#5 pcrman

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Posted 26 February 2011 - 10:32 AM

As Bob said, you hit a vein during injection. It is not a big deal. You can stop it by applying pressure to the injection site for 10-20 seconds.

#6 Limpa

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Posted 27 February 2011 - 06:58 PM

Thanks guys.
I know now. This is my first injection. Hopefully will improve soon.


Thanks




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