How to Handle Cells Shipped Internationally in Dry Ice?
Posted 24 February 2011 - 06:39 PM
I was wondering if anyone has experience importing frozen immortal, EBV-transformed lymphoblastoid cell lines from another country. For discussion's sake lets assume the cells will have been in dry-ice for at least 20 hours by the time they reach my lab. My questions are: How do I handle the cells when I receive them? Can they be stored at -80C or -20C directly or will that kill them? Should I instead thaw them out and immediately culture them? At what stage of thaw will the cells be at after being in dry-ice for 20-24 hours, assuming they were stored in liquid nitrogen prior to being packed for shipping?
Thanks very much.
Posted 25 February 2011 - 03:45 PM
Posted 01 March 2011 - 11:02 PM
I see. I presume that if there is metabolic activity without actual culturing, the cells may actually start dying off if not attended to. THank you.