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How to make tributyrin agar for lipid hydrolysis test ?


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#1 allymc

allymc

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Posted 24 February 2011 - 10:26 AM


Hello all-
I was wondering if someone could help with some basic media prep. I am trying to prepare some tributyrin agar plates for a lipid hydrolysis test. Is the agar base that is typically used a basic nutrient agar + tributyrin? I know the tributryin agar has to be mixed prior to pouring, are there any specific methods/tips? I read an article that indicated 10 ml per liter. Does this sound right?

Thanks in advance for any help!




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