What causes autofluorescence?
Posted 24 February 2011 - 09:48 AM
Today I noticed that cells from a mutant strain that I am using are more autofluorescent than the wild type. At first sight I thought of this as a big problem, but now I was wondering what can be leading the mutant cells to be more autofluorescent.
Importantly, I am talking about living, non-fixed cells.
Thanks in advance.
Posted 27 April 2011 - 07:16 AM
Posted 27 April 2011 - 07:38 AM
Posted 28 April 2011 - 12:27 AM
Apart from that, any big, organized structure can cause it, like Ribosomes, Actin filaments... Maybe you can help us by saying which cells you are working on and in which channel you find the increase.
Posted 30 April 2011 - 12:36 AM
I work with fungal cells and I find increased autofluorescence in all channels of the cytometer.
Posted 30 April 2011 - 06:42 AM