my question is, can we autoclave sodium bicarbonate and sodium carbonate?
from the protocol i saw, most of the people using filter sterilize method.
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#1
badguy
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Posted 23 February 2011 - 10:19 PM
Hi, i wanted to prepare carbonate buffer for my ELISA. it's my 1st time
my question is, can we autoclave sodium bicarbonate and sodium carbonate?
from the protocol i saw, most of the people using filter sterilize method.
my question is, can we autoclave sodium bicarbonate and sodium carbonate?
from the protocol i saw, most of the people using filter sterilize method.
#2
mdfenko
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an elder
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Posted 24 February 2011 - 12:11 PM
you should filter sterilize if you need it to be sterile.
but, why sterilize?
we make it fresh when we use it and don't sterilize.
but, why sterilize?
we make it fresh when we use it and don't sterilize.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
badguy
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Posted 24 February 2011 - 06:29 PM
mdfenko, on 24 February 2011 - 12:11 PM, said:
you should filter sterilize if you need it to be sterile.
but, why sterilize?
we make it fresh when we use it and don't sterilize.
but, why sterilize?
we make it fresh when we use it and don't sterilize.
can we autoclave it?
#4
mdfenko
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an elder
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Posted 25 February 2011 - 07:06 AM
i wouldn't. carbonate buffer is not very stable.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
