Posted 23 February 2011 - 05:49 PM
I have purified a protein and it appears a single band on a SDS-PAGE gel when stained with Coomassie blue. I need to know the purity of the protein. Often in many publications, I see many researchers mentioning similar to this "purity of the protein was >95% homogeneous as assessed from the SDS-PAGE gel". I know a software AlphaImager 2200, which density scan a lane and give the percent amount of all the proteins in a lane. But that was in previous lab, currently I am looking for a similar software available online for free. Or any other methods to identify the purity of the protein is also welcome.
thanks in advance