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Peptide transfection

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#1 Yasmin Samir Salah

Yasmin Samir Salah


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Posted 23 February 2011 - 07:59 AM

I am trying transform mammalian cells with plasmids, using an arginine peptide. I've read a few papers and seems like a successful method (also cheap).
However, one of the optimization steps is N/P charge ratio. In other words charge of peptide/DNA (which is ideally 3:1).
How do I calculate this?

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