help needed with intranasal administration in mice
Posted 23 February 2011 - 04:51 AM
Thanks in advance!
Posted 08 November 2012 - 01:24 AM
If you want to recruit neutrophils to the lungs, you're better off using a model of infection in the lungs. If you just want a way to recruit neutrophils for isolation, better to purify them from bone marrow, blood, or using a chemoattractant injected i.p. and lavaging the peritoneum. As for intranasal administrations, are the mice fully anaesthetised and breathing deeply when you do your i.n.s? That could be one issue if they are not asleep properly and not breathing the full dose in. Also, the i.n. technique doesn't actually reach all parts of the lung; only the main airways are exposed, the lower half of the airways gets very little exposure from an i.n..
Posted 08 November 2012 - 02:13 PM