Posted 23 February 2011 - 01:36 AM
I'm a newbie at using centrifugal concentrators for concentrating proteins.
Can anyone provide me with a basic step by step procedure?
My protein is lac repressor FPLC purifed using a cation exchanger column(P11-Phosphocellulose)
Posted 23 February 2011 - 02:10 AM
Posted 23 February 2011 - 03:07 AM
- add your sample to top container of the concentrator
- place in centrifuge and spin for certain amount of time (refer to instruction manual provided by the manufacturer)
- collect concentrated solution from the top container
Open this link: Amicon Ultra-15 Centrifugal Filter Units - then pick "User Guides" on the right (Related Resources section), then "Amicon Ultra-15 Centrifugal Filter Devices User Guide", then open PDF.
Posted 23 February 2011 - 03:18 AM
By the way, does anyone know how best to store the Lac repressor after centrifugal concentration?
Posted 04 March 2011 - 05:06 PM