16 replies to this topic

[Ameya P]

HI all,

On so many occasions, I have come across solutions to running your gels faster/ alternatives to slab gels etc etc. and now I dont even remember the names...

Basically, I am looking for something that will reduce electrophoresis time (ET) by a huge margin. I know there are  pre-cast gels that reduce the  ET to 15-20 mins. There is also something else, and does not even need a transilluminator. The only problem is, I dont remember the brand names.

All suggestions are welcome. (For all of those out there like me, send words that might even be remotely similar)

Thanks  

Ameya

[mdfenko]

are you thinking of the e-gel system by invitrogen?

[hobglobin]

another option would be Elchrom's Origin.

And there's always the option to use Lithium borate buffer to have faster gels in normal gel chambers...

[Ameya P]

mdfenko, on 23 February 2011 - 07:51 AM, said:

are you thinking of the e-gel system by invitrogen?

Yes something like this E gel. Would you know any other products, possibly cheaper alternatives.

I remember there was this system which had crimson red gels.


Thanks for your help.

[Adrian K]

What about Lonza:
http://www.lonza.com...s/flashgel.html

[Ameya P]

adrian kohsf, on 24 February 2011 - 03:04 AM, said:

What about Lonza:
http://www.lonza.com...s/flashgel.html

Adrian,

SPOT ON


Thanks!!!!      

[HomeBrew]

How many gels do you get for $105.00 USD?

[Adrian K]

gt_ameya, on 24 February 2011 - 08:54 PM, said:

adrian kohsf, on 24 February 2011 - 03:04 AM, said:

What about Lonza:
http://www.lonza.com...s/flashgel.html

Adrian,

SPOT ON


Thanks!!!!      

LOLx... welcome.... I forget the price but I remember is expensive...

[Ameya P]

HomeBrew, on 25 February 2011 - 01:36 AM, said:

How many gels do you get for $105.00 USD?

I dont know.... have asked for a quote.... please dont tell me ONE. I will never get the system then...

  + (tears in my eyes)...

[HomeBrew]

I'd appreciate it if you'd let us know...  Looking over their web site, I had the sinking feeling it was one gel.  The only indication I could find that it might not be was the description to the FlashGel DNA Starter Kit, which mentions it comes with "a pack of 1.2% DNA Cassettes"...

[Adrian K]

Hi HomeBrew, Ameya,
I can't remember much about the exact price and unit (the sales talk to me very long time ago) but I do barely remember the price for 1 piece is around ~RM40 (~USD10), the system which include the visualizing box is sold separately.

According to this website:
http://www.westburg....hgel-dna/$29816

It is sold in the packet of 9.

[HomeBrew]

Thanks adrian!

[Adrian K]

HomeBrew, on 27 February 2011 - 04:07 AM, said:

Thanks adrian!

You are welcome, HomeBrew... let us know how you think after you had tried it out...

[Ameya P]

HomeBrew, on 26 February 2011 - 08:37 AM, said:

I'd appreciate it if you'd let us know...  Looking over their web site, I had the sinking feeling it was one gel.  The only indication I could find that it might not be was the description to the FlashGel DNA Starter Kit, which mentions it comes with "a pack of 1.2% DNA Cassettes"...

I got quotes from Lonza and Invitrogen (E gel system).
Lonza : ~ $ 1800  
Invitrogen - $ 1300  

These are for the complete kit to get me started... Stupid gels can't be visulaized on a normal transilluminator...  now why would that be???   (They use EtBr)

adrian kohsf, on 26 February 2011 - 09:59 AM, said:

Hi HomeBrew, Ameya,
I can't remember much about the exact price and unit (the sales talk to me very long time ago) but I do barely remember the price for 1 piece is around ~RM40 (~USD10), the system which include the visualizing box is sold separately.

According to this website:
http://www.westburg....hgel-dna/$29816

It is sold in the packet of 9.

I am still waiting for their quotes on packs of 9 and 18... but at these rates of their starter kits... I cant hope for any relief. After speaking to my boss, I realized that the cost of running the usual agarose gels are as less $ 3   (that is rounding off to the NEXT INTEGER )

[Adrian K]

gt_ameya, on 04 March 2011 - 10:36 PM, said:

I am still waiting for their quotes on packs of 9 and 18... but at these rates of their starter kits... I cant hope for any relief. After speaking to my boss, I realized that the cost of running the usual agarose gels are as less $ 3   (that is rounding off to the NEXT INTEGER )

You mean USD 3?
For my calculation, my gel is around RM4, (USD1)
And yet, I most of the time still recycle my agarose by remelting in microwave and add EtBr again...