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How to reduce electrophoresis times


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16 replies to this topic

#1 Ameya P

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Posted 23 February 2011 - 12:18 AM

HI all,

On so many occasions, I have come across solutions to running your gels faster/ alternatives to slab gels etc etc. and now I dont even remember the names...

Basically, I am looking for something that will reduce electrophoresis time (ET) by a huge margin. I know there are pre-cast gels that reduce the ET to 15-20 mins. There is also something else, and does not even need a transilluminator. The only problem is, I dont remember the brand names.

All suggestions are welcome. (For all of those out there like me, send words that might even be remotely similar)

Thanks :)

Ameya

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#2 mdfenko

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Posted 23 February 2011 - 07:51 AM

are you thinking of the e-gel system by invitrogen?
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#3 hobglobin

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Posted 23 February 2011 - 09:18 AM

another option would be Elchrom's Origin.

And there's always the option to use Lithium borate buffer to have faster gels in normal gel chambers...
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#4 Ameya P

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Posted 23 February 2011 - 10:53 PM

are you thinking of the e-gel system by invitrogen?


Yes something like this E gel. Would you know any other products, possibly cheaper alternatives.

I remember there was this system which had crimson red gels.


Thanks for your help.

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#5 Adrian K

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Posted 24 February 2011 - 03:04 AM

What about Lonza:
http://www.lonza.com...s/flashgel.html
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#6 Ameya P

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Posted 24 February 2011 - 08:54 PM

What about Lonza:
http://www.lonza.com...s/flashgel.html


Adrian,

SPOT ON


Thanks!!!! :) :) :)

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#7 HomeBrew

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Posted 25 February 2011 - 01:36 AM

How many gels do you get for $105.00 USD?

#8 Adrian K

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Posted 25 February 2011 - 08:59 AM


What about Lonza:
http://www.lonza.com...s/flashgel.html


Adrian,

SPOT ON


Thanks!!!! :) :) :)



LOLx... welcome.... I forget the price but I remember is expensive...
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#9 Ameya P

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Posted 25 February 2011 - 09:12 PM

How many gels do you get for $105.00 USD?


I dont know.... have asked for a quote.... please dont tell me ONE. I will never get the system then...

:( + (tears in my eyes)...

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#10 HomeBrew

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Posted 26 February 2011 - 08:37 AM

I'd appreciate it if you'd let us know... Looking over their web site, I had the sinking feeling it was one gel. The only indication I could find that it might not be was the description to the FlashGel DNA Starter Kit, which mentions it comes with "a pack of 1.2% DNA Cassettes"...

#11 Adrian K

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Posted 26 February 2011 - 09:59 AM

Hi HomeBrew, Ameya,
I can't remember much about the exact price and unit (the sales talk to me very long time ago) but I do barely remember the price for 1 piece is around ~RM40 (~USD10), the system which include the visualizing box is sold separately.

According to this website:
http://www.westburg.eu/en/site/products/genomics-research/dna-gel-electrophoresis/lonza-flashgel-precast-gel-electrophoresis-systems/flashgel-dna/$29816

It is sold in the packet of 9.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#12 HomeBrew

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Posted 27 February 2011 - 04:07 AM

Thanks adrian!

#13 Adrian K

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Posted 27 February 2011 - 06:24 AM

Thanks adrian!

You are welcome, HomeBrew... let us know how you think after you had tried it out... ^_^
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#14 Ameya P

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Posted 04 March 2011 - 10:36 PM

I'd appreciate it if you'd let us know... Looking over their web site, I had the sinking feeling it was one gel. The only indication I could find that it might not be was the description to the FlashGel DNA Starter Kit, which mentions it comes with "a pack of 1.2% DNA Cassettes"...

I got quotes from Lonza and Invitrogen (E gel system).
Lonza : ~ $ 1800 :angry:
Invitrogen - $ 1300 :angry:

These are for the complete kit to get me started... Stupid gels can't be visulaized on a normal transilluminator... now why would that be??? :blink: (They use EtBr)

Hi HomeBrew, Ameya,
I can't remember much about the exact price and unit (the sales talk to me very long time ago) but I do barely remember the price for 1 piece is around ~RM40 (~USD10), the system which include the visualizing box is sold separately.

According to this website:
http://www.westburg.eu/en/site/products/genomics-research/dna-gel-electrophoresis/lonza-flashgel-precast-gel-electrophoresis-systems/flashgel-dna/$29816

It is sold in the packet of 9.


I am still waiting for their quotes on packs of 9 and 18... but at these rates of their starter kits... I cant hope for any relief. After speaking to my boss, I realized that the cost of running the usual agarose gels are as less $ 3 :unsure: (that is rounding off to the NEXT INTEGER )

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Replication of this art is strictly prohibited without express permission of the artist


#15 Adrian K

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Posted 04 March 2011 - 11:30 PM

I am still waiting for their quotes on packs of 9 and 18... but at these rates of their starter kits... I cant hope for any relief. After speaking to my boss, I realized that the cost of running the usual agarose gels are as less $ 3 :unsure: (that is rounding off to the NEXT INTEGER )


You mean USD 3?
For my calculation, my gel is around RM4, (USD1)
And yet, I most of the time still recycle my agarose by remelting in microwave and add EtBr again...
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434




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