I did a DNA extraction but now I can't dissolve the pellet in TE. I think I might have overdried the pellet. What can I do? The tubes are currently at 37º C, but no luck until now.
Thanks in advance.
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#1
cardosopedro
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Posted 22 February 2011 - 10:36 AM
Hi.
I did a DNA extraction but now I can't dissolve the pellet in TE. I think I might have overdried the pellet. What can I do? The tubes are currently at 37º C, but no luck until now.
Thanks in advance.
I did a DNA extraction but now I can't dissolve the pellet in TE. I think I might have overdried the pellet. What can I do? The tubes are currently at 37º C, but no luck until now.
Thanks in advance.
#2
mdfenko
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Posted 22 February 2011 - 10:49 AM
keep it at 37C and give it some time
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
nightingale
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Posted 23 February 2011 - 02:29 AM
Dissolve the DNA overnight on a shaker or at 55°C for 1–2 h.
this was what i did using Qiagen genomic DNA extraction kit.
this was what i did using Qiagen genomic DNA extraction kit.
" The more you learn, the more you realize how little you know ... "
#4
cardosopedro
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Posted 23 February 2011 - 03:13 AM
Thanks, I will try that right now!
