I need to determine transfection efficiency of a construct, and plan to do so using GFP expression. I will have uninfected (human)cells as a control, and am wondering what isotpye control I should use? I usually choose based on the antibody used for flow but since there is no antibody necessary, I was wondering should I just use a generic one- GFP anti-human IgG1 targeting?
Thanks in advance
Edited by phoebe75, 22 February 2011 - 09:15 AM.