2 replies to this topic

[phoebe75]

I need to determine transfection efficiency of a construct, and plan to do so using GFP expression. I will have uninfected (human)cells as a control, and am wondering what isotpye control I should use? I usually choose based on the antibody used for flow but since there is no antibody necessary, I was wondering should I just use a generic one-  GFP anti-human IgG1 targeting?
Thanks in advance

Edited by phoebe75, 22 February 2011 - 09:15 AM.

[leelee]

You shouldn't use an isotype control at all.

[phoebe75]

leelee, on 22 February 2011 - 07:05 PM, said:

You shouldn't use an isotype control at all.

Thanks for responding- that's what I thought and was just going to compare infected and uninfected cells, but then saw a couple of papers that showed results with isotype controls also. Will proceed as planned so- thanks again.