Trypsin for J774A Macrophages?
Posted 21 February 2011 - 01:52 PM
Posted 24 April 2012 - 07:13 PM
If you want to get them off the plate mildly, you can use 3 mM EDTA+10mM glucose in PBS too.
Posted 25 April 2012 - 08:00 AM
We grow our J774 in Techne stirrer bottles because:
They grow excellently in SUSPENSION
You do not need to trypsinise the cells to set up experiments
The stirrer bottles last for 20-30 years and can be autoclaved infinetely
The cells grow to 800,000 cells/ml and are 100% viable...so large quantities can be available for big experiments
The J774 cells stick like superglue to thye TC plastic so scraping is the only alternative....but viability can go down to 40-50% i.e. you lose over half the cells..........not cost effective at all
Spend the money and buy TECHNE stirrer bottles/stirrer platforms
Hope this helps