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Wavelength correction in ELISA


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#1 Crux

Crux

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Posted 21 February 2011 - 01:32 AM

Hi everyone!

I have a small question about an standard sandwich ELISA protocol. It says monitor color at 405nm with wavelength correction at 650nm. If the machine does not have such function, should I simply subtract values at 650nm from 405nm?

Thanks a lot!
Ying




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