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western without tween


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3 replies to this topic

#1 nataly

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Posted 21 February 2011 - 12:15 AM

hi,
I forgut to put tween while doing the western blot. will it change the results drastically?

#2 BioMiha

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Posted 21 February 2011 - 12:42 AM

Not really. You could observe slightly more non-specific binding but I wouldn't worry about it too much.

#3 little mouse

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Posted 21 February 2011 - 12:44 AM

hi,
I forgut to put tween while doing the western blot. will it change the results drastically?


you might have more background and/or non specific bands, or not, depending on your antibody.
good luck

#4 nataly

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Posted 21 February 2011 - 01:04 PM

thanks




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