hi,
I forgut to put tween while doing the western blot. will it change the results drastically?
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#2
BioMiha
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Posted 21 February 2011 - 12:42 AM
Not really. You could observe slightly more non-specific binding but I wouldn't worry about it too much.
#3
little mouse
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Posted 21 February 2011 - 12:44 AM
nataly, on 21 February 2011 - 12:15 AM, said:
hi,
I forgut to put tween while doing the western blot. will it change the results drastically?
I forgut to put tween while doing the western blot. will it change the results drastically?
you might have more background and/or non specific bands, or not, depending on your antibody.
good luck
