Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

LA-PCR issues


  • Please log in to reply
No replies to this topic

#1 Zach T

Zach T

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 18 February 2011 - 02:47 PM

So I am trying to amplify a ~13kb region of human DNA using LA-PCR. Currently I am using TaKaRa's kit and iProof by BioRad, but haven't been able to get a solid band on any of my gels. I was wondering if anyone else has had better luck with certain protocols or pcr programs/settings to get good amplification. Early on I was getting high smearing above 10kb. I've been messing with runtimes, adding betaine, and template concentrations but now I'm getting pretty much no amplification. Any comments would be appreciated, Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.