Posted 18 February 2011 - 08:25 AM
Hello everyone. I was wondering if any of you could offer some help on a cloning project I have going on. Basically, I'm trying to gel isolate a 3960 fragment from the gel. However, due to incomplete digestion I always end up with my linearized plasmid which is 4401 bp long. That's a difference of 441 base pairs. Last time I tried this project, I had to have help from the PhD student in our lab. I was wondering if anybody knew of any resources I could use to find out how the agarose % of a gel affects migration of the bands. For example, would a 2% gel allow for a larger distance between the two bands compared to a 1% gel (or vice versa)? Thank you for your input!
As our lab always says, "May the clone be with you."