nuclear extraction of blood cells
Posted 18 February 2011 - 05:06 AM
Here's my problem. I'm having some unexpected results in the detection of a nuclear protein. I suspect that this is related with the nuclear extraction step because, when I visualise the cells in the microscope, I see no swelling of the cells and lysis of the cytoplasmic membranes when it is supposed to happen. I have this problem in fresh human monocytes and neutrophils. So I was woundering if someone has a protocol of nuclear extraction that really works with this type of cells. Also, I would like to know if phosphatase inhibitors are essential to nuclear extraction because I've seen a protocol that uses them in the cell washing step but most protocols don't include them.
Thanks in advance.
Posted 19 May 2011 - 06:31 PM
Posted 19 May 2011 - 06:33 PM