Primary hippocampal neurons seem to be quite insensitive to glutamate treatment. Does anybody have a protocol treating them in magnesium-free, glycine containing medium to increases their sensitivity (disinhibtion of NMDA receptors)?
Best regards,
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scolix
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Posted 19 February 2011 - 08:45 AM
The time of the experiment can influence the toxicity. Hippocampal neuron are quite insensitive to glutamte within the first 5 days in culure.
This experiment is usually done in Locke's solution and you have to add glycine separately. The quality of these solutions can make a difference.
This experiment is usually done in Locke's solution and you have to add glycine separately. The quality of these solutions can make a difference.
