cloning a large insert
Posted 17 February 2011 - 08:01 PM
i am trying to clone a 7kb gene into a 6.2kb vector. I have tried different competent cell strains with no success. I also ran the ligation mix in an agarose gel and saw that ligation is working. How do i proceed with transforming this?
plss help. I am at my wit's end.
Posted 19 February 2011 - 08:55 AM
Posted 15 March 2011 - 02:35 AM
Posted 15 March 2011 - 02:45 AM
And if so: you kept that in mind making the calculations?
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 18 March 2011 - 08:12 PM
Posted 19 March 2011 - 05:42 AM