cloning a large insert
Posted 17 February 2011 - 08:01 PM
i am trying to clone a 7kb gene into a 6.2kb vector. I have tried different competent cell strains with no success. I also ran the ligation mix in an agarose gel and saw that ligation is working. How do i proceed with transforming this?
plss help. I am at my wit's end.
Posted 19 February 2011 - 08:55 AM
Posted 15 March 2011 - 02:35 AM
Posted 15 March 2011 - 02:45 AM
And if so: you kept that in mind making the calculations?
Posted 18 March 2011 - 08:12 PM
Posted 19 March 2011 - 05:42 AM