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succinate quantitation


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#1 AfBio

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Posted 17 February 2011 - 07:51 AM

Hi all. I'm new here. Does anybody know any way of succinate quantitation on mice liver extract? I've been trying with spectrophotometry at 340 nm using a kit that is usually used for food testing, but I'm not getting good results... Any idea?

#2 mdfenko

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Posted 17 February 2011 - 09:02 AM

you could adapt an assay with an enzyme that utilizes succinate such as succinic thiokinase:

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