I would like to know, please, if you know how feasible it would be to obtain a synaptosomal preparation from primary neurons. Would that yield enough material to detect a synaptic protein using WB, considering said protein is expressed in normal levels?
Also, does anyone know how tricky it is to obtain synaptodendrosomes after the synaptosomal preparation?
Thank you very much in advance for any suggestions/input.
Synaptosomal / synaptodendrosomal preparations
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