I am working on Total GIP(1-42).
I am having some difficulties with the peptide. I have an ELISA kit from Millipore company, and I have bought GIP peptide from a different company. The company has provided me the peptide in lyophilized form and I reconstituted it in water to make a concentration of 100ug/ml and then froze the aliquots. Now when I am running this peptide in the ELISA kit against my standard curve, it does not give me the expected concentration. The concentration is around 10 fold less and it varies when I repeat the experiment also the dilutions are not linear.
Can any of this behavior explained by the peptides structure, or it being hydrophobic or any other thing you can think of. I have tried talking to Millipore company but they do not have an insight about it.
Quantification of peptide Total GIP
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