How long can I store cleaved/digested vector?
Posted 16 February 2011 - 02:28 AM
Does anyone know how long I can store a vector that has been cleaved with restriction enzymes, analysed on gel and then purified and eluted in dH2O. The vector is stored at -20˚C. I know that linear DNA is not a stable as circular DNA. I would like to be able to use my restricted vector for ligation with a new PCR product, some weeks later then I did the restriction. Is that possible? How long can I store linear PCR product that has not been cleaved?
Posted 16 February 2011 - 05:18 AM
Posted 16 February 2011 - 05:38 AM
Posted 16 February 2011 - 06:57 AM
Posted 16 February 2011 - 06:47 PM
Posted 17 February 2011 - 04:56 AM
Posted 17 February 2011 - 08:11 AM
Posted 18 February 2011 - 05:12 AM
Posted 18 February 2011 - 05:29 AM
You can also switch to 0.1 mM EDTA in your TE, which is sometimes used instead of 1 mM, to reduce the effect of large amounts of DNA in reactions.