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restriction digestion


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#1 deespike

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Posted 16 February 2011 - 02:11 AM

Hi all

Can the restricted plasmid with overhangs stay stable when stored in -20C before using for cloning purpose? Do the overhangs get damaged by thawing once?

#2 jc545staffy

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Posted 16 February 2011 - 05:04 AM

constant freeze thawing can degrade the DNA but it should be ok once.
cells should be viable for up to a year at -70oC though




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