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why cut plasmid DNA with Hind111 and EcoR1?


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#1 jc545staffy

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Posted 15 February 2011 - 02:36 PM

Hi all

im new to this so if this has already been done and i havent searched for it sorry!

just woundering when preping plasmid DNA to analyse them with restriction enzymes why can we only use these ( in the title ) enzymes and not XHO1 or saL1 to visualise the inserts in pUC18??

thanks guys

#2 mdfenko

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Posted 16 February 2011 - 10:57 AM

probably because the inserts in the plasmids were made with those enzymes.
talent does what it can
genius does what it must
i do what i get paid to do




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