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Ruling out endogenous protein for interaction model (input)

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#1 Biochem_newbie



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Posted 15 February 2011 - 12:27 PM

Hi there

I am working on seeing if my protein of interest ( A ) is interacting with another protein of interest ( B ), but before I even try to understand the Co-IP results, I am having a hard time expressing the input. Sometimes it appears and sometimes it doesnt. I am guessing that one of my protein of interest ( B ) is affecting the expression of the second protein of interest ( A ) . In order to understand this hypothesis deeper, I am using a knockdown cell culture line ( B ).

I was wondering, why is it important to rule out endogenous protein ( B ) as a factor for the lack of expression for my protein of interest ( A )?

Thank you for any comments!

Edited by Biochem_newbie, 15 February 2011 - 12:28 PM.

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