Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Ruling out endogenous protein for interaction model (input)


  • Please log in to reply
No replies to this topic

#1 Biochem_newbie

Biochem_newbie

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 32 posts
0
Neutral

Posted 15 February 2011 - 12:27 PM

Hi there

I am working on seeing if my protein of interest ( A ) is interacting with another protein of interest ( B ), but before I even try to understand the Co-IP results, I am having a hard time expressing the input. Sometimes it appears and sometimes it doesnt. I am guessing that one of my protein of interest ( B ) is affecting the expression of the second protein of interest ( A ) . In order to understand this hypothesis deeper, I am using a knockdown cell culture line ( B ).

I was wondering, why is it important to rule out endogenous protein ( B ) as a factor for the lack of expression for my protein of interest ( A )?

Thank you for any comments!

Edited by Biochem_newbie, 15 February 2011 - 12:28 PM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.