5 replies to this topic

[Alvolo]

What is the proper way to infect cell culture with virus?
Some protocols mentioned to aspirate the virus inoculum after 2 hour of incubation and replace with fresh medium.
What will happen if we inoculate for longer period?

Edited by Alvolo, 15 February 2011 - 08:26 AM.

[bob1]

It depends on the aim of the experiment... if you do it as you described, you remove the possibility that the duration of the infection is from the virus you added, but if that is not a confounding factor, then you can leave the original virus on the culture.

[leelee]

Depends on your virus, depends on your experiment and also depends on the volume you are adding.

Can you give us some more details?

Edited to add:

Quote

What will happen if we inoculate for longer period?

Probably nothing (except perhaps your cells drying out due to evaporation, if you are infecting in a small volume). I think any virus that was able to enter your cells would have done so by this point so a longer incubation will probably have no effect.

Edited by leelee, 15 February 2011 - 07:52 PM.

[scolix]

As others might have suggested, you need to optimise for the type of virus and the experiment.

which virus are you using?

[Alvolo]

scolix, on 16 February 2011 - 10:47 AM, said:

As others might have suggested, you need to optimise for the type of virus and the experiment.

which virus are you using?

Thanks~ currently using ibdv.

[protolder]

Alvolo, on 16 February 2011 - 04:41 PM, said:

scolix, on 16 February 2011 - 10:47 AM, said:

As others might have suggested, you need to optimise for the type of virus and the experiment.

which virus are you using?

Thanks~ currently using ibdv.

Hola the idea of retire the inoculum is to sincrinize the infection, all the infected cells are infected at the same time. Buena suerte