2 replies to this topic

[PandaCreamPuff]

Hi all

I have been storing genomic DNA after a heat-lysis method in PBS. I have been doing this for some time and my PCR and sequencing seems to work fine. I am just worried that for my thesis this will cause me some mark deduction, as I did not use TE buffer, which is probably the conventional way of storing DNA. Has anyone stored gDNA in PBS?

Thanks

[bob1]

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.

[PandaCreamPuff]

bob1, on 15 February 2011 - 01:10 PM, said:

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.

Thanks Bob.