Storing genomic DNA in 4C with PBS?
Posted 15 February 2011 - 02:54 AM
I have been storing genomic DNA after a heat-lysis method in PBS. I have been doing this for some time and my PCR and sequencing seems to work fine. I am just worried that for my thesis this will cause me some mark deduction, as I did not use TE buffer, which is probably the conventional way of storing DNA. Has anyone stored gDNA in PBS?
Posted 15 February 2011 - 01:10 PM
Posted 17 February 2011 - 05:45 AM
It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.