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Storing genomic DNA in 4C with PBS?


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#1 PandaCreamPuff

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Posted 15 February 2011 - 02:54 AM

Hi all

I have been storing genomic DNA after a heat-lysis method in PBS. I have been doing this for some time and my PCR and sequencing seems to work fine. I am just worried that for my thesis this will cause me some mark deduction, as I did not use TE buffer, which is probably the conventional way of storing DNA. Has anyone stored gDNA in PBS?

Thanks

#2 bob1

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Posted 15 February 2011 - 01:10 PM

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.

#3 PandaCreamPuff

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Posted 17 February 2011 - 05:45 AM

It should be fine, this is more or less the pH and salt levels inside the cell anyway... however, they are more prone to bacterial/fungal and DNAse digestion if stored in PBS.


Thanks Bob.




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