Purification of His tagged protein
Posted 14 February 2011 - 11:29 PM
I am purify a his tagged protein expressed in E coli Bl 21DE3. But after gel filtration there are still contamination of different size of protein. I tried Ion exchange chromatography but it did not worked. Is there any thing i can do so that the unexpected protein will not be expressed or how to purify that one
Posted 15 February 2011 - 02:01 AM
Posted 15 February 2011 - 11:25 AM
but is the contamination a real problem for your exp.?