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Selection of SY5Y transfected cells with G418

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#1 Andreia Carvalho

Andreia Carvalho


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Posted 14 February 2011 - 07:14 AM


I did electroporation of my SY5Y cells and started the selection with G418 (400ug/mL)and the cells seemed ok. However, as the time goes by, they start to die. Should I lower the concentration of G418? Do you have any tip?

Thanks for your help.

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