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Differentiation of SY5Y cells


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#1 Andreia Carvalho

Andreia Carvalho

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Posted 14 February 2011 - 07:11 AM

Hello everyone!

I'm trying to differentiate SY5Y cells. The problem is that whenI add the all-trans retinoic acid (#R2625, Sigma) my cells die. I also used rall-trans retinal (#R2500, sigma) but the same happened. I'm using 104 cells/cm2. Can anyone help me? I don't know what is wrong with my protocol.

Thanks in advance.
A.Carvalho




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