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Micro-RNA profiling for differentiating cells


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#1 Drxian

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Posted 14 February 2011 - 01:15 AM

Hi everyone,

I am trying to profile the micro-RNAs related to differentiation of progenitor cells towards a particular cell type. I have been in discussion with the tech support of different companies regarding this. SABioscience has a pathway focused miRNA RT-RCR assay kit which focuses on 88 miRNAs and is supposedly focused on miRNAs related to cell differentiation and development. I do wish to use this product as it's easier than profiling the entire genome, however I am unable to find any literature which specifies why has the company focused on these particular 88 miRNAs and this is making me concerned a bit. I shall be glad if anyone can give me suggestions regarding this. I wish to look into the gene expression pattern in the early stages of differentiation, but am wondering as to what happens if I do not find any statistically different expression pattern for these 88 miRNAs and what would be a good approach for the project, if I encounter such a situation.

Thanks

Kind regards
Xian

#2 Drxian

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Posted 16 February 2011 - 08:28 PM

Hi everyone,

I would be really grateful for any suggestions you have

Kind regards
Nishant




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